CLIC1 Antibody

ProSci
Product Code: PSI-63-017
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-017-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CLIC1 antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
2 / 2
Western blot analysis of anti-CLIC1 Antibody in A375 cell line lysates (35ug/lane).

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CLIC1 antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of anti-CLIC1 Antibody in A375 cell line lysates (35ug/lane).

Further Information

Additional Names:
Chloride intracellular channel protein 1, Chloride channel ABP, Nuclear chloride ion channel 27, NCC27, Regulatory nuclear chloride ion channel protein, hRNCC, CLIC1, G6, NCC27
Application Note:
For IHC-P starting dilution is: 1:10~50

For WB starting dilution is: 1:1000
Background:
Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 1 is a member of the p64 family; the protein localizes principally to the cell nucleus and exhibits both nuclear and plasma membrane chloride ion channel activity.
Background References:
  • Singh,H., FEBS J. 274 (24), 6306-6316 (2007)
  • Ulmasov,B., (er) BMC Cell Biol. 8,(2007)
  • Edwards,J.C., J. Membr. Biol. 213 (1), 39-46 (2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This CLIC1 antibody is generated from rabbits immunized with a recombinant human CLIC1 protein.
NCBI Gene ID #:
1192
NCBI Official Name:
Chloride intracellular channel protein 1
NCBI Official Symbol:
CLIC1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
27 kDa
Protein Accession #:
O00299
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Obesity,Signal Transduction
Swissprot #:
O00299
User NOte:
Optimal dilutions for each application to be determined by the researcher.