HFE Antibody

ProSci
Product Code: PSI-62-313
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-313-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of HFE antibody in Jurkat cell line lysates (35ug/lane)
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HFE Antibody IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis of HFE antibody in Jurkat cell line lysates (35ug/lane)
HFE Antibody IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Hereditary hemochromatosis protein, HLA-H, HFE, HLAH
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
HFE is a membrane protein that is similar to MHC class I-type proteins and associates with beta2-microglobulin (beta2M). It is thought that this protein functions to regulate iron absorption by regulating the interaction of the transferrin receptor with transferrin. The iron storage disorder, hereditary haemochromatosis, is a recessive genetic disorder that results from defects in its gene.
Background References:
  • Valenti,L., ClinAm Soc Nephrol(8), 1331-1337 (2009)
  • Won,J.E., Intervirology 52 (5), 239-246 (2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This HFE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 122-148 amino acids from the Central region of human HFE.
NCBI Gene ID #:
3077
NCBI Official Name:
Hereditary hemochromatosis protein
NCBI Official Symbol:
HFE
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
40 kDa
Protein Accession #:
Q30201
Protein GI Number:
2497915
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Obesity,Signal Transduction
Swissprot #:
Q30201
User NOte:
Optimal dilutions for each application to be determined by the researcher.