PGK2 Antibody

ProSci
Product Code: PSI-62-701
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-701-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of anti-PGK2 in HepG2 cell line lysate (35ug/lane)
2 / 3
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
3 / 3

Western blot analysis of anti-PGK2 in HepG2 cell line lysate (35ug/lane)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.

Further Information

Additional Names:
Phosphoglycerate kinase 2, Phosphoglycerate kinase, testis specific, PGK2, PGKB
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
PGK2 is a form of phosphoglycerate kinase unique to spermatozoa. The autosomal PGK2 gene lacks introns and contains characteristics of a processed gene. The conservation of function in this processed PGK2 gene and its tissue-specific expression in spermatogenesis suggests that it exists as a compensatory response to the inactivation of the X-linked PGK1 gene in spermatogenic cells before meiosis.
Background References:
  • Zhang, L.P., et al., Biol. Reprod. 60(6):1329-1337 (1999).
  • McCarrey, J.R., et al., Dev. Genet. 19(4):321-332 (1996).
  • Blanche, H., et al., Genomics 9(3):420-428 (1991).
  • McCarrey, J.R., et al., Nature 326(6112):501-505 (1987).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Monkey
Immunogen:
This PGK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 117-145 amino acids from the N-terminal region of human PGK2.
NCBI Gene ID #:
5232
NCBI Official Name:
Phosphoglycerate kinase 2
NCBI Official Symbol:
PGK2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
45 kDa
Protein Accession #:
P07205
Protein GI Number:
21264485
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Obesity,Signal Transduction
Swissprot #:
P07205
User NOte:
Optimal dilutions for each application to be determined by the researcher.