IDH3B Antibody

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ProSci
Product Code: PSI-61-033
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-61-033-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Immunohistochemical analysis of paraffin-embedded H.brain section using IDH3B Antibody (N-term). Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Western blot analysis of lysates from Hela cell line and human placenta tissue lysate (from left to right), using IDH3B Antibody at 1:1000 at each lane.
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Immunohistochemical analysis of paraffin-embedded H.brain section using IDH3B Antibody (N-term). Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Western blot analysis of lysates from Hela cell line and human placenta tissue lysate (from left to right), using IDH3B Antibody at 1:1000 at each lane.

Documents

Further Information

Additional Names:
Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial, Isocitric dehydrogenase subunit beta, NAD(+)-specific ICDH subunit beta, IDH3B
Application Note:
For IHC-P starting dilution is: 1:25

For WB starting dilution is: 1:1000
Background References:
  • Kim Y.-O.,et al.J. Biol. Chem. 274:36866-36875(1999).
  • Ota T.,et al.Nat. Genet. 36:40-45(2004).
  • Deloukas P.,et al.Nature 414:865-871(2001).
  • Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Monkey
Immunogen:
This IDH3B antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 15-49 amino acids from the N-terminal region of human IDH3B.
NCBI Gene ID #:
3420
NCBI Official Name:
Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial
NCBI Official Symbol:
IDH3B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
42 kDa
Protein Accession #:
O43837
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Obesity,Signal Transduction
Swissprot #:
O43837
User NOte:
Optimal dilutions for each application to be determined by the researcher.