AGXT Antibody

ProSci
Product Code: PSI-62-279
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-279-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 7
Antibody staining AGXT in Human hepatic carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
2 / 7
Western Blot at 1:8000 dilution + human liver lysates Lysates/proteins at 20 ug per lane.
3 / 7
4 / 7
Western Blot at 1:2000 dilution + human liver lysates Lysates/proteins at 20 ug per lane.
5 / 7
Western Blot at 1:2000 dilution + human liver lysates Lysates/proteins at 20 ug per lane.
6 / 7
Western blot analysis of AGXT Antibody in HepG2 cell line lysates (35ug/lane)(8ug/ml)
7 / 7
AGXT Antibody (RB18848) IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Antibody staining AGXT in Human hepatic carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Western Blot at 1:8000 dilution + human liver lysates Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution + human liver lysates Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution + human liver lysates Lysates/proteins at 20 ug per lane.
Western blot analysis of AGXT Antibody in HepG2 cell line lysates (35ug/lane)(8ug/ml)
AGXT Antibody (RB18848) IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Serine--pyruvate aminotransferase, SPT, Alanine--glyoxylate aminotransferase, AGT, AGXT, AGT1, SPAT
Application Note:
For IHC-P starting dilution is: 1:25

For WB starting dilution is: 1:1000
Background:
AGXT is expressed only in the liver and protein is localized mostly in the peroxisomes, where it is involved in glyoxylate detoxification.
Background References:
  • Cellini,B., J. Biol. Chem. 284 (13), 8349-8358 (2009)
  • Bertoldi,M., Biochim. Biophys. Acta 1784 (9), 1356-1362 (2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This AGXT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 96-125 amino acids from the Central region of human AGXT.
NCBI Gene ID #:
189
NCBI Official Name:
Serine--pyruvate aminotransferase
NCBI Official Symbol:
AGXT
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
43 kDa
Protein Accession #:
P21549
Protein GI Number:
134855
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Obesity,Signal Transduction
Swissprot #:
P21549
User NOte:
Optimal dilutions for each application to be determined by the researcher.