GFPT2 Antibody

ProSci
Product Code: PSI-63-873
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-873-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of GFPT2 Antibody in A2058, K562 cell line lysates (35ug/lane)
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GFPT2 Antibody IHC analysis in formalin fixed and paraffin embedded human skin followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis of GFPT2 Antibody in A2058, K562 cell line lysates (35ug/lane)
GFPT2 Antibody IHC analysis in formalin fixed and paraffin embedded human skin followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 2, D-fructose-6-phosphate amidotransferase 2, Glutamine:fructose-6-phosphate amidotransferase 2, GFAT 2, GFAT2, Hexosephosphate aminotransferase 2, GFPT2
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
GFPT2 controls the flux of glucose into the hexosamine pathway. This protein most likely involved in regulating the availability of precursors for N-and O-linked glycosylation of proteins.
Background References:
  • Edwards,T.L., et.al., Ann. Hum. Genet. 74 (2), 97-109 (2010)
  • Prasad,P., et.al., BMC Med. Genet. 11, 52 (2010)
  • Srinivasan,V., et.al., Clin. Biochem. 40 (13-14), 952-957 (2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat
Immunogen:
This GFPT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 175-201 amino acids from the Central region of human GFPT2.
NCBI Gene ID #:
9945
NCBI Official Name:
Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 2
NCBI Official Symbol:
GFPT2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
77 kDa
Protein Accession #:
O94808
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Obesity,Signal Transduction
Swissprot #:
O94808
User NOte:
Optimal dilutions for each application to be determined by the researcher.