ASS1 Antibody

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ProSci
Product Code: PSI-56-532
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-56-532-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of ASS1 using rabbit polyclonal ASS1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ASS1 gene.
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Western blot analysis in Jurkat cell line lysates (35ug/lane).
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Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis of ASS1 using rabbit polyclonal ASS1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ASS1 gene.
Western blot analysis in Jurkat cell line lysates (35ug/lane).
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Documents

Further Information

Additional Names:
Argininosuccinate synthase, Citrulline--aspartate ligase, ASS1, ASS
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
The protein encoded by this gene catalyzes the penultimate step of the arginine biosynthetic pathway. There are approximately 10 to 14 copies of this gene including the pseudogenes scattered across the human genome, among which the one located on chromosome 9 appears to be the only functional gene for argininosuccinate synthetase. Mutations in the chromosome 9 copy of ASS cause citrullinemia. Two transcript variants encoding the same protein have been found for this gene.
Background References:
  • Hozyasz, K.K., et al. Arch. Oral Biol. 55(11):861-866(2010)
  • Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
  • Kobayashi, E., et al. Mol. Cancer Ther. 9(3):535-544(2010)
  • Tsai, W.B., et al. Mol. Cancer Ther. 8(12):3223-3233(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Mouse, Rat
Immunogen:
This ASS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 281-310 amino acids from the C-terminal region of human ASS1.
NCBI Gene ID #:
445
NCBI Official Name:
Argininosuccinate synthase
NCBI Official Symbol:
ASS1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
47 kDa
Protein Accession #:
P00966
Protein GI Number:
20141195
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Obesity,Signal Transduction
Swissprot #:
P00966
User NOte:
Optimal dilutions for each application to be determined by the researcher.