AGPAT3 Antibody

ProSci
Product Code: PSI-63-587
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-587-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of AGPAT3 Antibody in A2058 cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human brain tissue reacted with AGPAT3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of AGPAT3 Antibody in A2058 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human brain tissue reacted with AGPAT3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Further Information

Additional Names:
1-acyl-sn-glycerol-3-phosphate acyltransferase gamma, 1-acylglycerol-3-phosphate O-acyltransferase 3, 1-AGP acyltransferase 3, 1-AGPAT 3, Lysophosphatidic acid acyltransferase gamma, LPAAT-gamma, AGPAT3, LPAAT3
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
AGPAT3 is an acyltransferase that converts lysophosphatidic acid into phosphatidic acid, which is the second step in the de novo phospholipid biosynthetic pathway. This protein may be an integral membrane protein.
Background References:
  • Leung,D.W. et.al., Front. Biosci. 6, D944-D953 (2001)
  • Lu,B., et.al., Biochem. J. 385 (PT 2), 469-477 (2005)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This AGPAT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 241-269 amino acids from the Central region of human AGPAT3.
NCBI Gene ID #:
56894
NCBI Official Name:
1-acyl-sn-glycerol-3-phosphate acyltransferase gamma
NCBI Official Symbol:
AGPAT3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
43 kDa
Protein Accession #:
Q9NRZ7
Protein GI Number:
12643817
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Obesity,Signal Transduction
Swissprot #:
Q9NRZ7
User NOte:
Optimal dilutions for each application to be determined by the researcher.