ATP5H Antibody
| Code | Size | Price |
|---|
| PSI-55-073-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Fluorescence-activated cell sorting (FACS)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
ATP synthase subunit d, mitochondrial, ATPase subunit d, ATP5H
Application Note:
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
For WB starting dilution is: 1:1000
Background:
Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the d subunit of the Fo complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. In addition, three pseudogenes are located on chromosomes 9, 12 and 15.
Background References:
- Martins-de-Souza, D., et al.Psychiatr Res 43(11):978-986(2009) Kim, D.W., et al. Cancer Sci. 99(10):1884-1891(2008) Cross, R.L. Nature 427(6973):407-408(2004) Oster, G., et al. Trends Cell Biol. 13(3):114-121(2003) Leyva, J.A., et al. Mol. Membr. Biol. 20(1):27-33(2003)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This ATP5H antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68-97 amino acids from the Central region of human ATP5H.
NCBI Gene ID #:
10476
NCBI Official Name:
ATP synthase subunit d, mitochondrial
NCBI Official Symbol:
ATP5H
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
18 kDa
Protein Accession #:
O75947
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Obesity,Signal Transduction
Swissprot #:
O75947
User NOte:
Optimal dilutions for each application to be determined by the researcher.