ENASE Antibody

ProSci
Product Code: PSI-55-593
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-55-593-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in HL-60 cell line lysates (35ug/lane).
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ENASE Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in HL-60 cell line lysates (35ug/lane).
ENASE Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Cytosolic endo-beta-N-acetylglucosaminidase, ENGase, ENGASE
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
Endo-beta-N-acetylglucosaminidase (ENGase; EC 3.2.1.96) is involved in the processing of free oligosaccharides in the cytosol.
Background References:
  • Park, K., et al. Diabetes 59(7):1845-1850(2010)
  • Dorfmueller, H.C., et al. FEBS Lett. 584(4):694-700(2010)
  • Chantret, I., et al. PLoS ONE(7), E11734 (2010) :
  • Butkinaree, C., et al. J. Biol. Chem. 283(35):23557-23566(2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This ENASE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 326-354 amino acids from the Central region of human ENASE.
NCBI Gene ID #:
64772
NCBI Official Name:
Cytosolic endo-beta-N-acetylglucosaminidase
NCBI Official Symbol:
ENGASE
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
84 kDa
Protein Accession #:
Q8NFI3
Protein GI Number:
74715557
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Obesity,Signal Transduction
Swissprot #:
Q8NFI3
User NOte:
Optimal dilutions for each application to be determined by the researcher.