PECI Antibody

ProSci
Product Code: PSI-63-592
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-592-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of PECI Antibody in HepG2 cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PECI Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of PECI using rabbit polyclonal PECI Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PECI gene.

Western blot analysis of PECI Antibody in HepG2 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PECI Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of PECI using rabbit polyclonal PECI Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PECI gene.

Further Information

Additional Names:
Enoyl-CoA delta isomerase 2, mitochondrial, DRS-1, Delta(3),delta(2)-enoyl-CoA isomerase, D3,D2-enoyl-CoA isomerase, Diazepam-binding inhibitor-related protein 1, DBI-related protein 1, Dodecenoyl-CoA isomerase, Hepatocellular carcinoma-associated antigen 88, Peroxisomal 3,2-trans-enoyl-CoA isomerase, pECI, Renal carcinoma antigen NY-REN-1, ECI2, DRS1, HCA88, PECI
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
PECI is an auxiliary enzyme that catalyzes an isomerization step required for the beta-oxidation of unsaturated fatty acids.
Background References:
  • Scanlan,M.J., et.al., Int. J. Cancer 83 (4), 456-464 (1999)
  • Suk,K., et.al., Biochim. Biophys. Acta 1454 (1), 126-131 (1999)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PECI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 331-358 amino acids from the C-terminal region of human PECI.
NCBI Gene ID #:
10455
NCBI Official Name:
Enoyl-CoA delta isomerase 2, mitochondrial
NCBI Official Symbol:
ECI2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
44 kDa
Protein Accession #:
O75521
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Obesity,Signal Transduction
Swissprot #:
O75521
User NOte:
Optimal dilutions for each application to be determined by the researcher.