AKR1B1 Antibody

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ProSci
Product Code: PSI-61-465
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-61-465-400ul400ul£662.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in A375 cell line and human placenta tissue lysates (35ug/lane).This demonstrates the AKR1B1 antibody detected the AKR1B1 protein (arrow).
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Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with AKR1B1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis in A375 cell line and human placenta tissue lysates (35ug/lane).This demonstrates the AKR1B1 antibody detected the AKR1B1 protein (arrow).
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with AKR1B1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Documents

Further Information

Additional Names:
Aldose reductase, AR, Aldehyde reductase, Aldo-keto reductase family 1 member B1, AKR1B1, ALDR1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50
Background:
AKR1B1 is a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This protein catalyzes the reduction of a number of aldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol.
Background References:
  • Steuber,H.,J. Mol. Biol. 379 (5), 991-1016 (2008)
  • Gleissner,C.A.,Arterioscler. Thromb. Vasc. Biol. 28 (6), 1137-1143 (2008)
  • Grundmann,U.,DNA Cell Biol.(3), 149-157 (1990)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This AKR1B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 290-316 amino acids from the C-terminal region of human AKR1B1.
NCBI Gene ID #:
231
NCBI Official Name:
Aldose reductase
NCBI Official Symbol:
AKR1B1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
36 kDa
Protein Accession #:
P15121
Protein GI Number:
113596
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Obesity,Signal Transduction
Swissprot #:
P15121
User NOte:
Optimal dilutions for each application to be determined by the researcher.