GGH Antibody

ProSci
Product Code: PSI-56-199
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-56-199-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
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GGH Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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4 / 4
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
GGH Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Gamma-glutamyl hydrolase, Conjugase, GH, Gamma-Glu-X carboxypeptidase, GGH
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
This gene catalyzes the hydrolysis of folylpoly-gamma-glutamates and antifolylpoly-gamma-glutamates by the removal of gamma-linked polyglutamates and glutamate. [provided by RefSeq].
Background References:
  • Adjei, A.A., et al.Thorac Oncol 5(9):1346-1353(2010)
  • Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010)
  • Organista-Nava, J., et al. Leuk. Res. 34(6):728-732(2010)
  • Figueiredo, J.C., et al. Cancer Causes Control 21(4):597-608(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This GGH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 229-256 amino acids from the C-terminal region of human GGH.
NCBI Gene ID #:
8836
NCBI Official Name:
Gamma-glutamyl hydrolase
NCBI Official Symbol:
GGH
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
36 kDa
Protein Accession #:
Q92820
Protein GI Number:
6016127
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Obesity,Signal Transduction
Swissprot #:
Q92820
User NOte:
Optimal dilutions for each application to be determined by the researcher.