ADH1B Antibody

ProSci
Product Code: PSI-62-438
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-438-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:2000 dilution Lane 1: human liver lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.
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Western blot analysis of ADH1B Antibody in HepG2 cell line lysates (35ug/lane)
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Flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Confocal immunofluorescent analysis of ADH1B Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western Blot at 1:2000 dilution Lane 1: human liver lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of ADH1B Antibody in HepG2 cell line lysates (35ug/lane)
Flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of ADH1B Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Further Information

Additional Names:
Alcohol dehydrogenase 1B, Alcohol dehydrogenase subunit beta, ADH1B, ADH2
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50
Background:
The protein is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. This encoded protein, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism.
Background References:
  • Alcohol intake, Am. J. Gastroenterol. 104 (9), 2182-2188 (2009) Nishimura,F.T., Nihon Arukoru Yakubutsu Igakkai Zasshi 44 (3), 139-155 (2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Chicken, Mouse, Rat
Immunogen:
This ADH1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 209-237 amino acids from the Central region of human ADH1B.
NCBI Gene ID #:
125
NCBI Official Name:
Alcohol dehydrogenase 1B
NCBI Official Symbol:
ADH1B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
40 kDa
Protein Accession #:
P00325
Protein GI Number:
113394
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Obesity,Signal Transduction
Swissprot #:
P00325
User NOte:
Optimal dilutions for each application to be determined by the researcher.