TFAM Antibody

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ProSci
Product Code: PSI-55-117
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-55-117-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of lysates from Hela, HepG2, U-2OS cell line (from left to right), using TFAM Antibody at 1:1000 at each lane.
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Western blot analysis of lysate from 293T cell line, using TFAM Antibody at 1:1000.
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Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Fluorescent confocal image of NCI-H460 cell stained with TFAM Antibody . NCI-H460 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TFAM primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).TFAM immunoreactivity is localized to mitochondrion significantly.
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Western blot analysis in Hela,Jurkat,K562,MCF-7 cell line lysates (35ug/lane).
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Western blot analysis of lysates from Hela, HepG2, U-2OS cell line (from left to right), using TFAM Antibody at 1:1000 at each lane.
Western blot analysis of lysate from 293T cell line, using TFAM Antibody at 1:1000.
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Fluorescent confocal image of NCI-H460 cell stained with TFAM Antibody . NCI-H460 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TFAM primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).TFAM immunoreactivity is localized to mitochondrion significantly.
Western blot analysis in Hela,Jurkat,K562,MCF-7 cell line lysates (35ug/lane).

Documents

Further Information

Additional Names:
Transcription factor A, mitochondrial, mtTFA, Mitochondrial transcription factor 1, MtTF1, Transcription factor 6, TCF-6, Transcription factor 6-like 2, TFAM, TCF6, TCF6L2
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50
Background:
This gene encodes a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. Studies in mice have demonstrated that this gene product is required to regulate the mitochondrial genome copy number and is essential for embryonic development. A mouse model for Kearns-Sayre syndrome was produced when expression of this gene was eliminated by targeted disruption in heart and muscle cells.
Background References:
  • Wang, W., et al. Nucleic Acids Res. (2010) In press :
  • Corneveaux, J.J., et al. Hum. Mol. Genet. 19(16):3295-3301(2010)
  • Akhmetov, I.I., et al. Fiziol Cheloveka 36(2):121-125(2010)
  • Shulman, J.M., et al. PLoS ONE(6), E11244 (2010) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This TFAM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 216-246 amino acids from the C-terminal region of human TFAM.
NCBI Gene ID #:
7019
NCBI Official Name:
Transcription factor A, mitochondrial
NCBI Official Symbol:
TFAM
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
29 kDa
Protein Accession #:
Q00059
Protein GI Number:
417324
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Obesity,Signal Transduction
Swissprot #:
Q00059
User NOte:
Optimal dilutions for each application to be determined by the researcher.