ATP5O Antibody

ProSci
Product Code: PSI-63-519
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-519-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of ATP5O Antibody in 293 cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human skeletal muscle with ATP5O Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Flow cytometric analysis of 293 cells using ATP5O Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis of ATP5O using rabbit polyclonal ATP5O Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ATP5O gene.

Western blot analysis of ATP5O Antibody in 293 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human skeletal muscle with ATP5O Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of 293 cells using ATP5O Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of ATP5O using rabbit polyclonal ATP5O Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ATP5O gene.

Further Information

Additional Names:
ATP synthase subunit O, mitochondrial, Oligomycin sensitivity conferral protein, OSCP, ATP5O, ATPO
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For IF starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
ATP5O is a component of the F-type ATPase found in the mitochondrial matrix. F-type ATPases are composed of a catalytic core and a membrane proton channel. This protein appears to be part of the connector linking these two components and may be involved in transmission of conformational changes or proton conductance.
Background References:
  • Wang,L., et.al., Cancer Epidemiol. Biomarkers Prev. 17 (12), 3558-3566 (2008)
  • Contessi,S., et.al., J. Bioenerg. Biomembr. 39 (4), 291-300 (2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Pig
Immunogen:
This ATP5O antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 38-64 amino acids from the N-terminal region of human ATP5O.
NCBI Gene ID #:
539
NCBI Official Name:
ATP synthase subunit O, mitochondrial
NCBI Official Symbol:
ATP5O
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
23 kDa
Protein Accession #:
P48047
Protein GI Number:
1352049
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Obesity,Signal Transduction
Swissprot #:
P48047
User NOte:
Optimal dilutions for each application to be determined by the researcher.