EPS15 Antibody
| Code | Size | Price |
|---|
| PSI-61-119-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Epidermal growth factor receptor substrate 15, Protein Eps15, Protein AF-1p, EPS15, AF1P
Application Note:
For WB starting dilution is: 1:1000
Background:
EPS15 is involved in cell growth regulation, possibly via the regulation of mitogenic signals and control of cell proliferation. EPS15 also participates in the internalization of ligand-inducible receptors of the receptor tyrosine kinase (RTK) type, in particular EGFR. Potential interaction partners include AP2A2, STN2, EPN1, and CRK via its SH3-binding sites. EPS15 is ubiquitously expressed. Phosphorylation on Tyr-849 is involved in the internalization of EGFR. EPS15 is not required for membrane translocation after EGF treatment or for targeting to coated pits, but essential for a subsequent step in EGFR endocytosis. This protein is involved in a t(1;11)(p32;q23) chromosomal translocation in acute leukemias causing fusion to the trithorax (MLL or HRX) gene product which contains DNA-binding motifs resulting in a rogue activator protein. Structurally, EPS15 contains 2 EF-hand calcium-binding domains, 3 EH domains, and 2 ubiquitin-interacting motif (UIM) repeats.
Background References:
- de Beer, T., et al., Nat. Struct. Biol. 7(11):1018-1022 (2000).
- Enmon, J.L., et al., Biochemistry 39(15):4309-4319 (2000).
- de Beer, T., et al., Science 281(5381):1357-1360 (1998).
- Chen, H., et al., Nature 394(6695):793-797 (1998).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This EPS15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 840-870 amino acids from the C-terminal region of human EPS15.
NCBI Gene ID #:
2060
NCBI Official Name:
Epidermal growth factor receptor substrate 15
NCBI Official Symbol:
EPS15
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
99 kDa
Protein Accession #:
P42566
Protein GI Number:
67476728
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction
Swissprot #:
P42566
User NOte:
Optimal dilutions for each application to be determined by the researcher.