PDAP1 Antibody

ProSci
Product Code: PSI-62-202
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-202-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Antibody is used in Western blot to detect PDAP1 in HL60 lysate.
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Western blot analysis of PDAP1 using rabbit polyclonal PDAP1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDAP1 gene (Lane 2).

Antibody is used in Western blot to detect PDAP1 in HL60 lysate.
Western blot analysis of PDAP1 using rabbit polyclonal PDAP1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDAP1 gene (Lane 2).

Further Information

Additional Names:
28 kDa heat- and acid-stable phosphoprotein, PDGF-associated protein, PAP, PDGFA-associated protein 1, PAP1, PDAP1, HASPP28
Application Note:
For WB starting dilution is: 1:1000
Background:
Human PDAP1 encodes a deduced 181-amino acid protein expressed in all tissues test with the exception of liver, most abundantly in brain. PDAP1 enhances PDGFA-stimulated cell growth in mouse fibroblasts, but inhibits the mitogenic effect of PDGFB. The binding between PDGFA and PDAP1 is a low affinity/high capacity interaction, as determined by sold-phase studies.
Background References:
  • Fischer, W.H., et al., J. Neurochem. 66(5):2213-2216 (1996).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This PDAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 142-171 amino acids from the C-terminal region of human PDAP1.
NCBI Gene ID #:
11333
NCBI Official Name:
28 kDa heat- and acid-stable phosphoprotein
NCBI Official Symbol:
PDAP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
21 kDa
Protein Accession #:
Q13442
Protein GI Number:
2498464
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction
Swissprot #:
Q13442
User NOte:
Optimal dilutions for each application to be determined by the researcher.