AP2B1 Antibody

ProSci
Product Code: PSI-58-131
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-58-131-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in A2058 cell line lysates (35ug/lane).

Western blot analysis in A2058 cell line lysates (35ug/lane).

Further Information

Additional Names:
AP-2 complex subunit beta, AP105B, Adaptor protein complex AP-2 subunit beta, Adaptor-related protein complex 2 subunit beta, Beta-2-adaptin, Beta-adaptin, Clathrin assembly protein complex 2 beta large chain, Plasma membrane adaptor HA2/AP2 adaptin beta subunit, AP2B1, ADTB2, CLAPB1
Application Note:
For WB starting dilution is: 1:1000
Background:
The protein encoded by this gene is one of two large chain components of the assembly protein complex 2, which serves to link clathrin to receptors in coated vesicles. The encoded protein is found on the cytoplasmic face of coated vesicles in the plasma membrane. Two transcript variants encoding different isoforms have been found for this gene.
Background References:
  • Kahlfeldt, N., et al. J. Biol. Chem. 285(4):2734-2749(2010)
  • Hood, F.E., et al. J. Cell. Sci. 122 (PT 13), 2185-2190 (2009) :
  • Grass, B., et al. Histopathology 54(7):873-879(2009)
  • Keyel, P.A., et al. Mol. Biol. Cell 19(12):5309-5326(2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat
Immunogen:
This AP2B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 517-546 amino acids from the Central region of human AP2B1.
NCBI Gene ID #:
163
NCBI Official Name:
AP-2 complex subunit beta
NCBI Official Symbol:
AP2B1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
105 kDa
Protein Accession #:
P63010
Protein GI Number:
51702211
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
P63010
User NOte:
Optimal dilutions for each application to be determined by the researcher.