PI4K2A Antibody

ProSci
Product Code: PSI-58-816
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-58-816-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: HL-60 whole cell lysate Lysates/proteins at 20 ug per lane.
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Western blot analysis in CEM cell line lysates (35ug/lane).

Western Blot at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: HL-60 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in CEM cell line lysates (35ug/lane).

Further Information

Additional Names:
Phosphatidylinositol 4-kinase type 2-alpha, Phosphatidylinositol 4-kinase type II-alpha, PI4K2A
Application Note:
For WB starting dilution is: 1:1000
Background:
Phosphatidylinositolpolyphosphates (PtdInsPs) are centrally involved in many biologic processes, ranging from cell growth and organization of the actin cytoskeleton to endo- and exocytosis. PI4KII phosphorylates PtdIns at the D-4 position, an essential step in the biosynthesis of PtdInsPs (Barylko et al., 2001 [PubMed 11244087]).
Background References:
  • Li, J., et al. Oncogene 29(17):2550-2559(2010)
  • Qin, Y., et al. J. Biol. Chem. 284(34):22544-22548(2009)
  • Salazar, G., et al. J. Biol. Chem. 284(3):1790-1802(2009)
  • Craige, B., et al. Mol. Biol. Cell 19(4):1415-1426(2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Zebrafish, Rat
Immunogen:
This PI4K2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 387-415 amino acids from the C-terminal region of human PI4K2A.
NCBI Gene ID #:
55361
NCBI Official Name:
Phosphatidylinositol 4-kinase type 2-alpha
NCBI Official Symbol:
PI4K2A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
54 kDa
Protein Accession #:
Q9BTU6
Protein GI Number:
74752344
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q9BTU6
User NOte:
Optimal dilutions for each application to be determined by the researcher.