SRP14 Antibody

ProSci
Product Code: PSI-60-309
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-60-309-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:2000 dilution Lane 1: A375 whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.

Western Blot at 1:2000 dilution Lane 1: A375 whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.

Further Information

Additional Names:
Signal recognition particle 14 kDa protein, SRP14, 18 kDa Alu RNA-binding protein, SRP14
Application Note:
For WB starting dilution is: 1:2000
Background:
Signal-recognition-particle assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane. SRP9 together with SRP14 and the Alu portion of the SRP RNA, constitutes the elongation arrest domain of SRP. The complex of SRP9 and SRP14 is required for SRP RNA binding.
Background References:
  • Mary, C., et al. RNA 16(5):969-979(2010)
  • Lakkaraju, A.K., et al. Cell 133(3):440-451(2008)
  • Yoshida, M., et al. Mol. Pharmacol. 73(3):987-994(2008)
  • Lamesch, P., et al. Genomics 89(3):307-315(2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Monkey
Immunogen:
This SRP14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 28-55 amino acids from the Central region of human SRP14.
NCBI Gene ID #:
6727
NCBI Official Name:
Signal recognition particle 14 kDa protein
NCBI Official Symbol:
SRP14
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
15 kDa
Protein Accession #:
P37108
Protein GI Number:
116242801
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
P37108
User NOte:
Optimal dilutions for each application to be determined by the researcher.