ATP6V1G1 Antibody

ProSci
Product Code: PSI-60-605
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-60-605-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in ZR-75-1 cell line lysates (35ug/lane).

Western blot analysis in ZR-75-1 cell line lysates (35ug/lane).

Further Information

Additional Names:
V-type proton ATPase subunit G 1, V-ATPase subunit G 1, V-ATPase 13 kDa subunit 1, Vacuolar proton pump subunit G 1, Vacuolar proton pump subunit M16, ATP6V1G1, ATP6G, ATP6G1, ATP6J
Application Note:
For WB starting dilution is: 1:1000
Background:
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. The protein encoded by this gene is one of three V1 domain G subunit proteins. Pseudogenes of this gene have been characterized.
Background References:
  • Norgett, E.E., et al. J. Biol. Chem. 282(19):14421-14427(2007)
  • Lamesch, P., et al. Genomics 89(3):307-315(2007)
  • Stelzl, U., et al. Cell 122(6):957-968(2005)
  • Morel, N. Biol. Cell 95(7):453-457(2003)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse
Immunogen:
This ATP6V1G1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 90-118 amino acids from the C-terminal region of human ATP6V1G1.
NCBI Gene ID #:
9550
NCBI Official Name:
V-type proton ATPase subunit G 1
NCBI Official Symbol:
ATP6V1G1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
14 kDa
Protein Accession #:
O75348
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
O75348
User NOte:
Optimal dilutions for each application to be determined by the researcher.