PPEF1 Antibody

ProSci
Product Code: PSI-57-703
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-57-703-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of PPEF1 using rabbit polyclonal PPEF1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PPEF1 gene.
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Western blot analysis in CEM cell line lysates (35ug/lane).

Western blot analysis of PPEF1 using rabbit polyclonal PPEF1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PPEF1 gene.
Western blot analysis in CEM cell line lysates (35ug/lane).

Further Information

Additional Names:
Serine/threonine-protein phosphatase with EF-hands 1, PPEF-1, Protein phosphatase with EF calcium-binding domain, PPEF, Serine/threonine-protein phosphatase 7, PP7, PPEF1, PPEF, PPP7C
Application Note:
For WB starting dilution is: 1:1000
Background:
This gene encodes a member of the serine/threonine protein phosphatase with EF-hand motif family. The protein contains a protein phosphatase catalytic domain, and at least two EF-hand calcium-binding motifs in its C terminus. Although its substrate(s) is unknown, the encoded protein has been suggested to play a role in specific sensory neuron function and/or development. This gene shares high sequence similarity with the Drosophila retinal degeneration C (rdgC) gene. Several alternatively spliced transcript variants, each encoding a distinct isoform, have been described.
Background References:
  • Ross, M.T., et al. Nature 434(7031):325-337(2005)
  • Hillman, R.T., et al. Genome Biol.(2), R8 (2004) :
  • Gevaert, K., et al. Nat. Biotechnol. 21(5):566-569(2003)
  • Kutuzov, M.A., et al. Biochem. Biophys. Res. Commun. 293(3):1047-1052(2002)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PPEF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 298-327 amino acids from the Central region of human PPEF1.
NCBI Gene ID #:
5475
NCBI Official Name:
Serine/threonine-protein phosphatase with EF-hands 1
NCBI Official Symbol:
PPEF1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
76 kDa
Protein Accession #:
O14829
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
O14829
User NOte:
Optimal dilutions for each application to be determined by the researcher.