AT8B2 Antibody

ProSci
Product Code: PSI-55-414
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-55-414-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in CEM cell line lysates (35ug/lane).
2 / 2
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis in CEM cell line lysates (35ug/lane).
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Phospholipid-transporting ATPase ID, ATPase class I type 8B member 2, P4-ATPase flippase complex alpha subunit ATP8B2, ATP8B2, ATPID, KIAA1137
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of aminophospholipid-transporting ATPases. The aminophospholipid translocases transport phosphatidylserine and phosphatidylethanolamine from one side of a bilayer to another. Alternatively spliced transcript variants encoding different isoforms have been identified.
Background References:
  • Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
  • Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
  • Harris, M.J., et al. Biochim. Biophys. Acta 1633(2):127-131(2003)
  • Halleck, M.S., et al. Physiol. Genomics 1(3):139-150(1999)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This AT8B2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 18-47 amino acids from the N-terminal region of human AT8B2.
NCBI Gene ID #:
57198
NCBI Official Name:
Phospholipid-transporting ATPase ID
NCBI Official Symbol:
ATP8B2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
137 kDa
Protein Accession #:
P98198
Protein GI Number:
30316371
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
P98198
User NOte:
Optimal dilutions for each application to be determined by the researcher.