SIA8C Antibody

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ProSci
Product Code: PSI-61-983
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-61-983-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in HL-60 cell line lysates (15ug/lane).
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Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis in HL-60 cell line lysates (15ug/lane).
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Documents

Further Information

Additional Names:
Sia-alpha-2,3-Gal-beta-1,4-GlcNAc-R:alpha 2,8-sialyltransferase, 2499-, Alpha-2,8-sialyltransferase 8C, Alpha-2,8-sialyltransferase III, ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 3, Sialyltransferase 8C, SIAT8-C, Sialyltransferase St8Sia III, ST8SiaIII, ST8SIA3, SIAT8C
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
ST8SIA3 belongs to a family of sialyltransferases that form sialyl-alpha-2,8-sialyl-R linkages at the nonreducing termini of glycoconjugates (Lee et al., 1998 [PubMed 9826427]).
Background References:
  • Fellay, J., et al. PLoS Genet.(12), E1000791 (2009) :
  • Kim, S.J., et al. Biochem. Biophys. Res. Commun. 344(4):1057-1064(2006)
  • Angata, K., et al. J. Biol. Chem. 275(24):18594-18601(2000)
  • Lee, Y.C., et al. Arch. Biochem. Biophys. 360(1):41-46(1998)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This SIA8C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 312-340 amino acids from the C-terminal region of human SIA8C.
NCBI Gene ID #:
51046
NCBI Official Name:
Sia-alpha-2,3-Gal-beta-1,4-GlcNAc-R:alpha 2,8-sialyltransferase
NCBI Official Symbol:
ST8SIA3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
44 kDa
Protein Accession #:
O43173
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
O43173
User NOte:
Optimal dilutions for each application to be determined by the researcher.