NUP35 Antibody

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ProSci
Product Code: PSI-56-703
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-56-703-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in Hela cell line lysates (35ug/lane).
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Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis in Hela cell line lysates (35ug/lane).
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Documents

Further Information

Additional Names:
Nucleoporin NUP53, 35 kDa nucleoporin, Mitotic phosphoprotein 44, MP-44, Nuclear pore complex protein Nup53, Nucleoporin Nup35, NUP35, MP44, NUP53
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
This gene encodes a member of the nucleoporin family. The protein is localized to the nuclear rim and is part of the nuclear pore complex (NPC). All molecules entering or leaving the nucleus either diffuse through or are actively transported by the NPC.
Background References:
  • Ichikawa, S., et al. J. Bone Miner. Res. 25(8):1821-1829(2010)
  • Hawryluk-Gara, L.A., et al. Mol. Biol. Cell 19(4):1753-1762(2008)
  • Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
  • Lamesch, P., et al. Genomics 89(3):307-315(2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This NUP35 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 289-318 amino acids from the C-terminal region of human NUP35.
NCBI Gene ID #:
129401
NCBI Official Name:
Nucleoporin NUP53
NCBI Official Symbol:
NUP35
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
35 kDa
Protein Accession #:
Q8NFH5
Protein GI Number:
74730292
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q8NFH5
User NOte:
Optimal dilutions for each application to be determined by the researcher.