SFRP4 Antibody

ProSci
Product Code: PSI-55-922
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-55-922-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in K562 cell line lysates (35ug/lane).
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Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis in K562 cell line lysates (35ug/lane).
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Secreted frizzled-related protein 4, sFRP-4, Frizzled protein, human endometrium, FrpHE, SFRP4, FRPHE
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. The expression of SFRP4 in ventricular myocardium correlates with apoptosis related gene expression.
Background References:
  • Lee, D.Y., et al. Menopause 17(5):1064-1070(2010)
  • Kohno, H., et al. Oncol. Rep. 24(2):423-431(2010)
  • Frank, B., et al. Carcinogenesis 31(8):1381-1386(2010)
  • Huang, D., et al. J. Cancer Res. Clin. Oncol. 136(3):395-401(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This SFRP4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 318-346 amino acids from the C-terminal region of human SFRP4.
NCBI Gene ID #:
6424
NCBI Official Name:
Secreted frizzled-related protein 4
NCBI Official Symbol:
SFRP4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
40 kDa
Protein Accession #:
Q6FHJ7
Protein GI Number:
61216780
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q6FHJ7
User NOte:
Optimal dilutions for each application to be determined by the researcher.