NIP7 Antibody

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ProSci
Product Code: PSI-63-591
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-591-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.
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Flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western Blot at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.
Flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Documents

Further Information

Additional Names:
60S ribosome subunit biogenesis protein NIP7 homolog, KD93, NIP7
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
Required for proper 27S pre-rRNA processing and 60S ribosome subunit assembly.
Background References:
  • Liu,J.F., et.al., J. Struct. Biol. 148 (3), 370-374 (2004)
  • Chagnon,P., et.al., Am. J. Hum. Genet. 71 (6), 1443-1449 (2002)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Pig
Immunogen:
This NIP7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 150-177 amino acids from the C-terminal region of human NIP7.
NCBI Gene ID #:
51388
NCBI Official Name:
60S ribosome subunit biogenesis protein NIP7 homolog
NCBI Official Symbol:
NIP7
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
20 kDa
Protein Accession #:
Q9Y221
Protein GI Number:
56404676
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q9Y221
User NOte:
Optimal dilutions for each application to be determined by the researcher.