DNAJC9 Antibody

ProSci
Product Code: PSI-56-746
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-56-746-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in Jurkat cell line lysates (35ug/lane).
2 / 2
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis in Jurkat cell line lysates (35ug/lane).
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
DnaJ homolog subfamily C member 9, DnaJ protein SB73, DNAJC9
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
DNAJC9 is upregulated at both mRNA and protein levels after various mitogenic and stress stimulations. DNAJC9 is mainly localized in cell nuclei under normal culture conditions, and transported into cytoplasm and plasma membrane upon heat shock stress through a non-classical and lipid-dependent pathway. DNAJC9 can interact with HSP70s and activate the ATPase activity of HSP70s, both of which are dependent on the J domain. Research has indicated that DNAJC9 is a novel cochaperone for HSP70s.
Background References:
  • Rose, J. Phd, et al. Mol. Med. (2010) In press :
  • Pan, Z., et al. Neoplasma 57(2):123-128(2010)
  • Han, C., et al. Biochem. Biophys. Res. Commun. 353(2):280-285(2007)
  • Takamura, Y., et al. Biochem. Biophys. Res. Commun. 285(2):387-392(2001)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This DNAJC9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 29-58 amino acids from the N-terminal region of human DNAJC9.
NCBI Gene ID #:
23234
NCBI Official Name:
DnaJ homolog subfamily C member 9
NCBI Official Symbol:
DNAJC9
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
30 kDa
Protein Accession #:
Q8WXX5
Protein GI Number:
52782787
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q8WXX5
User NOte:
Optimal dilutions for each application to be determined by the researcher.