KRT25 Antibody

ProSci
Product Code: PSI-55-737
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-55-737-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in K562 cell line lysates (35ug/lane).
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Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis in K562 cell line lysates (35ug/lane).
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Keratin, type I cytoskeletal 25, Cytokeratin-25, CK-25, Keratin-25, K25, Keratin-25A, K25A, Type I inner root sheath-specific keratin-K25irs1, KRT25 (HGNC:30839)
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
This gene encodes a member of the type I (acidic) keratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. The type I keratin genes are clustered in a region of chromosome 17q12-q21.
Background References:
  • Rose, J. Phd, et al. Mol. Med. (2010) In press :
  • Schweizer, J., et al. J. Cell Biol. 174(2):169-174(2006)
  • Rogers, M.A., et al. Differentiation 72 (9-10), 527-540 (2004) :
  • Hesse, M., et al. Eur. J. Cell Biol. 83(1):19-26(2004)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This KRT25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 380-408 amino acids from the C-terminal region of human KRT25.
NCBI Gene ID #:
147183
NCBI Official Name:
Keratin, type I cytoskeletal 25
NCBI Official Symbol:
KRT25
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
49 kDa
Protein Accession #:
Q7Z3Z0
Protein GI Number:
74723316
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q7Z3Z0
User NOte:
Optimal dilutions for each application to be determined by the researcher.