CLDN15 Antibody

ProSci
Product Code: PSI-64-137
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-64-137-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of CLDN15 Antibody in K562 cell line lysates (35ug/lane)
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Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of CLDN15 Antibody in K562 cell line lysates (35ug/lane)
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Claudin-15, CLDN15
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
Plays a major role in tight junction specific obliteration of the intercellular space, through calcium independent cell adhesion activity
Background References:
  • Van Itallie, C.M., et al. Am. J. Physiol. Renal Physiol. 285 (6), F1078-F1084 (2003)
  • Gonzalez-Mariscal, L., et al. Prog. Biophys. Mol. Biol. 81(1):1-44(2003)
  • Tsukita, S., et al. Curr. Opin. Cell Biol. 14(5):531-536(2002)
  • Colegio, O.R., et al. Am. J. Physiol., Cell Physiol. 283 (1), C142-C147 (2002)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This CLDN15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 128-157 amino acids from the Central region of human CLDN15.
NCBI Gene ID #:
24146
NCBI Official Name:
Claudin-15
NCBI Official Symbol:
CLDN15
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
24 kDa
Protein Accession #:
P56746
Protein GI Number:
6685306
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
P56746
User NOte:
Optimal dilutions for each application to be determined by the researcher.