BLZF1 Antibody

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ProSci
Product Code: PSI-64-020
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-64-020-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Fluorescent image of A549 cell stained with BLZF1 Antibody .A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with BLZF1 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml).BLZF1 immunoreactivity is localized to Nucleus significantly.
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Western blot analysis of BLZF1 Antibody in HepG2 cell line lysates (35ug/lane)
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Fluorescent image of A549 cell stained with BLZF1 Antibody .A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with BLZF1 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml).BLZF1 immunoreactivity is localized to Nucleus significantly.
Western blot analysis of BLZF1 Antibody in HepG2 cell line lysates (35ug/lane)

Documents

Further Information

Additional Names:
Golgin-45, Basic leucine zipper nuclear factor 1, JEM-1, p45 basic leucine-zipper nuclear factor, BLZF1, JEM1
Application Note:
For IF starting dilution is: 1:10~50

For WB starting dilution is: 1:1000
Background:
Required for normal Golgi structure and for protein transport from the endoplasmic reticulum (ER) through the Golgi apparatus to the cell surface.
Background References:
  • Ehret, G.B., et al. Eur. J. Hum. Genet. 17(12):1650-1657(2009)
  • Cheung, C.L., et al. Hum. Mol. Genet. 18(4):679-687(2009)
  • Reichman, T.W., et al. Mol. Cell. Biol. 22(1):343-356(2002)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This BLZF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 156-184 amino acids from the Central region of human BLZF1.
NCBI Gene ID #:
8548
NCBI Official Name:
Golgin-45
NCBI Official Symbol:
BLZF1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
45 kDa
Protein Accession #:
Q9H2G9
Protein GI Number:
116242501
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q9H2G9
User NOte:
Optimal dilutions for each application to be determined by the researcher.