CRTAP Antibody

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ProSci
Product Code: PSI-58-911
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-58-911-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Fluorescent image of Hela cells stained with CRTAP Antibody (N-term). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
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Western blot analysis of lysates from HT-1080, ZR-75-1 cell line (from left to right), using CRTAP Antibody at 1:1000 at each lane.
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Fluorescent image of Hela cells stained with CRTAP Antibody (N-term). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Western blot analysis of lysates from HT-1080, ZR-75-1 cell line (from left to right), using CRTAP Antibody at 1:1000 at each lane.

Documents

Further Information

Additional Names:
Cartilage-associated protein, CRTAP, CASP
Application Note:
For IF starting dilution is: 1:25

For WB starting dilution is: 1:1000
Background:
The protein encoded by this gene is similar to the chicken and mouse CRTAP genes. The encoded protein is a scaffolding protein that may influence the activity of at least one member of the cytohesin/ARNO family in response to specific cellular stimuli. Defects in this gene are associated with osteogenesis imperfecta, a connective tissue disorder characterized by bone fragility and low bone mass.
Background References:
  • Morello, R., et al. Curr Osteoporos Rep 8(2):77-83(2010)
  • Li, G.H., et al. Osteoporos Int 21(6):1009-1020(2010)
  • Fratzl-Zelman, N., et al. Bone 46(3):820-826(2010)
  • Chang, W., et al. Hum. Mol. Genet. 19(2):223-234(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This CRTAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 31-59 amino acids from the N-terminal region of human CRTAP.
NCBI Gene ID #:
10491
NCBI Official Name:
Cartilage-associated protein
NCBI Official Symbol:
CRTAP
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
47 kDa
Protein Accession #:
O75718
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
O75718
User NOte:
Optimal dilutions for each application to be determined by the researcher.