NRK Antibody

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ProSci
Product Code: PSI-57-051
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-57-051-400ul400ul£626.00
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in A549 cell line lysates (35ug/lane).
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Fluorescent confocal image of Hela cell stained with NRK Antibody .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NRK primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NRK immunoreactivity is localized to Cytoplasm and Nucleus significantly.
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Western blot analysis in A549 cell line lysates (35ug/lane).
Fluorescent confocal image of Hela cell stained with NRK Antibody .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NRK primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NRK immunoreactivity is localized to Cytoplasm and Nucleus significantly.

Documents

Further Information

Additional Names:
Nik-related protein kinase, NRK
Application Note:
For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50
Background:
The mouse ortholog of this gene encodes a protein kinase required for JNK activation. The encoded protein may be involved in the induction of actin polymerization in late embryogenesis.
Background References:
  • Ross, M.T., et al. Nature 434(7031):325-337(2005)
  • Nakano, K., et al. Exp. Cell Res. 287(2):219-227(2003)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This NRK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 721-750 amino acids from the Central region of human NRK.
NCBI Gene ID #:
203447
NCBI Official Name:
Nik-related protein kinase
NCBI Official Symbol:
NRK
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
178 kDa
Protein Accession #:
Q7Z2Y5
Protein GI Number:
115502506
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q7Z2Y5
User NOte:
Optimal dilutions for each application to be determined by the researcher.