ELP2 Antibody

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ProSci
Product Code: PSI-61-576
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-61-576-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Fluorescent confocal image of SY5Y cells stained with ELP2 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with ELP2 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the ELP2 immunosignal mainly to the cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000134759).
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Western blot analysis of ELP2 antibody in NCI-H460 cell line lysates (35ug/lane)
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Fluorescent confocal image of SY5Y cells stained with ELP2 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with ELP2 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the ELP2 immunosignal mainly to the cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000134759).
Western blot analysis of ELP2 antibody in NCI-H460 cell line lysates (35ug/lane)

Documents

Further Information

Additional Names:
Elongator complex protein 2, ELP2, SHINC-2, STAT3-interacting protein 1, StIP1, ELP2, STATIP1
Application Note:
For IF starting dilution is: 1:100

For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
ELP2 regulates the ligand-dependent activation of STAT3. The protein acts as subunit of the RNA polymerase II elongator complex, which is a histone acetyltransferase component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. Elongator may play a role in chromatin remodeling and is involved in acetylation of histones H3 and probably H4.
Background References:
  • References for protein:
  • Kim,J.H., Proc. Natl. Acad. Sci. U.S.A. 99 (3), 1241-1246 (2002)
  • Hawkes,N.A., J. Biol. Chem. 277 (4), 3047-3052 (2002)
  • References for SY5Y (SH-SY5Y; ATCCCRL-2266): 1. Ross RA, et al. Coordinate morphological and biochemical interconversion of human neuroblastoma cells. J. Natl. Cancer Inst. 71: 741-749, 1983. [PubMed: 6137586]; 2. Biedler JL, et al. Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones. Cancer Res. 38: 3751-3757, 1978. [PubMed: 29704].
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This ELP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 737-765 amino acids from the C-terminal region of human ELP2.
NCBI Gene ID #:
55250
NCBI Official Name:
Elongator complex protein 2
NCBI Official Symbol:
ELP2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
93 kDa
Protein Accession #:
Q6IA86
Protein GI Number:
83305834
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction
Swissprot #:
Q6IA86
User NOte:
Optimal dilutions for each application to be determined by the researcher.