ITGA8 Antibody

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ProSci
Product Code: PSI-55-928
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-55-928-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in K562 cell line lysates (35ug/lane).
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ITGA8 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in K562 cell line lysates (35ug/lane).
ITGA8 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Documents

Further Information

Additional Names:
Integrin alpha-8, Integrin alpha-8 heavy chain, Integrin alpha-8 light chain, ITGA8
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
Integrin alpha-8/beta-1 functions in the genesis of kidney and probably of other organs by regulating the recruitment of mesenchymal cells into epithelial structures. It recognizes the sequence R-G-D in a wide array of ligands including TNC, FN1, SPP1 TGFB1, TGFB3 and VTN. NPNT is probably its functional ligand in kidney genesis. Neuronal receptor for TNC it mediates cell-cell interactions and regulates neurite outgrowth of sensory and motor neurons.
Background References:
  • Benoit, Y.D., et al. Biochem. Biophys. Res. Commun. 399(3):434-439(2010)
  • Simon-Sanchez, J., et al. Nat. Genet. 41(12):1308-1312(2009)
  • Benoit, Y.D., et al. Biol. Cell 101(12):695-708(2009)
  • Sato, Y., et al. J. Biol. Chem. 284(21):14524-14536(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This ITGA8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1025-1053 amino acids from the C-terminal region of human ITGA8.
NCBI Gene ID #:
8516
NCBI Official Name:
Integrin alpha-8
NCBI Official Symbol:
ITGA8
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
117 kDa
Protein Accession #:
P53708
Protein GI Number:
311033437
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
P53708
User NOte:
Optimal dilutions for each application to be determined by the researcher.