WNT16 Antibody

ProSci
Product Code: PSI-56-929
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-56-929-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of lysates from human brain, mouse brain tissue (from left to right), using WNT16 Antibody at 1:1000 at each lane.
2 / 3
Western blot analysis in CEM cell line lysates (35ug/lane).
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Western blot analysis of lysates from human brain, mouse brain tissue (from left to right), using WNT16 Antibody at 1:1000 at each lane.
Western blot analysis in CEM cell line lysates (35ug/lane).

Further Information

Additional Names:
Protein Wnt-16, WNT16
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It contains two transcript variants diverging at the 5' termini. These two variants are proposed to be the products of separate promoters and not to be splice variants from a single promoter. They are differentially expressed in normal tissues, one of which (variant 2) is expressed at significant levels only in the pancreas, whereas another one (variant 1) is expressed more ubiquitously with highest levels in adult kidney, placenta, brain, heart, and spleen.
Background References:
  • Binet, R., et al. Cancer Res. 69(24):9183-9191(2009)
  • Memarian, A., et al. Leuk. Lymphoma 50(12):2061-2070(2009)
  • Nygren, M.K., et al. Exp. Hematol. 37(2):225-233(2009)
  • Teh, M.T., et al. J. Cell. Sci. 120 (PT 2), 330-339 (2007) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This WNT16 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 236-265 amino acids from the C-terminal region of human WNT16.
NCBI Gene ID #:
51384
NCBI Official Name:
Protein Wnt-16
NCBI Official Symbol:
WNT16
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
41 kDa
Protein Accession #:
Q9UBV4
Protein GI Number:
12643875
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q9UBV4
User NOte:
Optimal dilutions for each application to be determined by the researcher.