MLKLAK Antibody

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ProSci
Product Code: PSI-63-376
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-63-376-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in HepG2 cell line lysate (35ug/lane). MLKLAK-C284(arrow) was detected using the purified Pab. This Western blot detected isoform 2 of MLKLAK, the accession number for MLKLAK isoform 1 is Q9NYL2, NP_057737.
2 / 2
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
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Western blot analysis in HepG2 cell line lysate (35ug/lane). MLKLAK-C284(arrow) was detected using the purified Pab. This Western blot detected isoform 2 of MLKLAK, the accession number for MLKLAK isoform 1 is Q9NYL2, NP_057737.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.

Documents

Further Information

Additional Names:
Mitogen-activated protein kinase kinase kinase MLT, Human cervical cancer suppressor gene 4 protein, HCCS-4, Leucine zipper- and sterile alpha motif-containing kinase, MLK-like mitogen-activated protein triple kinase, Mixed lineage kinase-related kinase, MLK-related kinase, MRK, Sterile alpha motif- and leucine zipper-containing kinase AZK, ZAK, MLTK
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
MLKLAK is a member of the MAPKKK family of signal transduction molecules. It possesses an N-terminal kinase catalytic domain, followed by a leucine zipper motif and a sterile-alpha motif (SAM). This magnesium-binding protein forms homodimers and is located in the cytoplasm. The protein mediates gamma radiation signaling leading to cell cycle arrest and activity of this protein plays a role in cell cycle checkpoint regulation in cells. The protein also has pro-apoptotic activity.
Background References:
  • Blume-Jensen P, et al. Nature 2001. 411: 355.
  • Cantrell D, J. Cell Sci. 2001. 114: 1439.
  • JhiangOncogene 2000. 19: 5590.
  • Manning G, et al. Science 2002. 298: 1912.
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This MLKLAK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 271-300 amino acids from the Central region of human MLKLAK.
NCBI Gene ID #:
51776
NCBI Official Name:
Mitogen-activated protein kinase kinase kinase MLT
NCBI Official Symbol:
ZAK
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
91 kDa
Protein Accession #:
Q9NYL2
Protein GI Number:
313104215
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction
Swissprot #:
Q9NYL2
User NOte:
Optimal dilutions for each application to be determined by the researcher.