TNK1 Antibody

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ProSci
Product Code: PSI-63-169
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-63-169-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of anti-TNK1 pab in Hela cell line lysate. Dilution of anti-TNK1 was 1:100; dilution of secondary antibody (goat anti-rabbit-HRP) was 1:7000. Data and protocol courtesy of Dr. Richard Lu, Partners HealthCare System at Harvard University.
2 / 2
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
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Western blot analysis of anti-TNK1 pab in Hela cell line lysate. Dilution of anti-TNK1 was 1:100; dilution of secondary antibody (goat anti-rabbit-HRP) was 1:7000. Data and protocol courtesy of Dr. Richard Lu, Partners HealthCare System at Harvard University.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.

Documents

Further Information

Additional Names:
Non-receptor tyrosine-protein kinase TNK1, CD38 negative kinase 1, TNK1 {ECO:0000312|EMBL:AAC994121}
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.
Background References:
  • Blume-Jensen P, et al. Nature 2001. 411: 355.
  • Cantrell D, J. Cell Sci. 2001. 114: 1439.
  • JhiangOncogene 2000. 19: 5590.
  • Manning G, et al. Science 2002. 298: 1912.
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This TNK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 256-286 amino acids from the Central region of human TNK1.
NCBI Gene ID #:
8711
NCBI Official Name:
Non-receptor tyrosine-protein kinase TNK1
NCBI Official Symbol:
TNK1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
72 kDa
Protein Accession #:
Q13470
Protein GI Number:
116242821
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction
Swissprot #:
Q13470
User NOte:
Optimal dilutions for each application to be determined by the researcher.