KRT6C Antibody

ProSci
Product Code: PSI-57-552
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-57-552-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in A549 cell line lysates (35ug/lane).
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KRT6C Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human esophagus carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis in A549 cell line lysates (35ug/lane).
KRT6C Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human esophagus carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Keratin, type II cytoskeletal 6C, Cytokeratin-6C, CK-6C, Cytokeratin-6E, CK-6E, Keratin K6h, Keratin-6C, K6C, Type-II keratin Kb12, KRT6C, KRT6E
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into epithelial keratins and hair keratins. The type II keratins are clustered in a region of chromosome 12q13. [provided by RefSeq].
Background References:
  • Bowden, P.E. J. Invest. Dermatol. 130(2):336-338(2010)
  • Wilson, N.J., et al. J. Invest. Dermatol. 130(2):425-429(2010)
  • Schweizer, J., et al. J. Cell Biol. 174(2):169-174(2006)
  • Scherer, S.E., et al. Nature 440(7082):346-351(2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This KRT6C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 135-164 amino acids from the N-terminal region of human KRT6C.
NCBI Gene ID #:
286887
NCBI Official Name:
Keratin, type II cytoskeletal 6C
NCBI Official Symbol:
KRT6C
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
60 kDa
Protein Accession #:
P48668
Protein GI Number:
59803089
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
P48668
User NOte:
Optimal dilutions for each application to be determined by the researcher.