PSKH1 Antibody

ProSci
Product Code: PSI-62-708
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-708-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of anti-PSKH1 Antibody (N-term H70) in Hela cell line lysate and mouse kidney tissue lysate (35ug/lane)
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot analysis of anti-PSKH1 Antibody (N-term H70) in Hela cell line lysate and mouse kidney tissue lysate (35ug/lane)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.

Further Information

Additional Names:
Serine/threonine-protein kinase H1, Protein serine kinase H1, PSK-H1, PSKH1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
PSKH1 may be a SFC-associated serine kinase (splicing factor compartment-associated serine kinase) with a role in intranuclear SR protein (non-snRNP splicing factors containing a serine/arginine-rich domain) trafficking and pre-mRNA processing. PSKH1 is localized in the Brefeldin A-sensitive Golgi compartment, at centrosomes, in the nucleus with a somewhat speckle-like presence, membrane-associated to the endoplasmic reticulum (ER) and the plasma membrane (PM), and more diffusely in the cytoplasm.
Background References:
  • Brede, G., et al., Exp. Cell Res. 291(2):299-312 (2003).
  • Brede, G., et al., Nucleic Acids Res. 30(23):5301-5309 (2002).
  • Brede, G., et al., Genomics 70(1):82-92 (2000).
  • Amarzguioui, M., et al., Nucleic Acids Res. 28(21):4113-4124 (2000).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This PSKH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 55-91 amino acids from the N-terminal region of human PSKH1.
NCBI Gene ID #:
5681
NCBI Official Name:
Serine/threonine-protein kinase H1
NCBI Official Symbol:
PSKH1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
48 kDa
Protein Accession #:
P11801
Protein GI Number:
17379144
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction
Swissprot #:
P11801
User NOte:
Optimal dilutions for each application to be determined by the researcher.