PLS1 Antibody

ProSci
Product Code: PSI-56-881
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-56-881-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in Jurkat cell line lysates (35ug/lane).
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PLS1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis in Jurkat cell line lysates (35ug/lane).
PLS1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Plastin-1, Intestine-specific plastin, I-plastin, PLS1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. The protein encoded by this gene is a third distinct plastin isoform, which is specifically expressed at high levels in the small intestine. Alternatively spliced transcript variants varying in the 5' UTR, but encoding the same protein, have been found for this gene. A pseudogene of this gene is found on chromosome 11.
Background References:
  • Chafel, M.M., et al. Dev. Dyn. 203(2):141-151(1995)
  • Lin, C.S., et al. Mol. Cell. Biol. 14(4):2457-2467(1994)
  • Shibata, M., et al. J. Leukoc. Biol. 54(1):10-16(1993)
  • Lin, C.S., et al. J. Biol. Chem. 268(4):2781-2792(1993)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PLS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 550-579 amino acids from the C-terminal region of human PLS1.
NCBI Gene ID #:
5357
NCBI Official Name:
Plastin-1
NCBI Official Symbol:
PLS1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
70 kDa
Protein Accession #:
Q14651
Protein GI Number:
224471848
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q14651
User NOte:
Optimal dilutions for each application to be determined by the researcher.