DKK2 Antibody
| Code | Size | Price |
|---|
| PSI-58-168-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Dickkopf-related protein 2, Dickkopf-2, Dkk-2, hDkk-2, DKK2
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For IHC-P starting dilution is: 1:50~100
Background:
The 259-amino acid DKK2 protein, like DKK1, DKK3, and DKK4, possesses an N-terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a linker region and contain 10 cys residues each. The second cys region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis revealed expression of 4.0- and 4.5-kb DKK2 transcripts in heart, brain, skeletal muscle, and lung. Western blot analysis showed that DKK2 is secreted as a 15- to 17-kD protein. Functional analysis determined that DKK2 does not block Xenopus Wnt8 induction of a secondary axis in frog embryos.
Background References:
- Clark, H.F., et al., Genome Res. 13(10):2265-2270 (2003).
- Brott, B.K., et al., Mol. Cell. Biol. 22(17):6100-6110 (2002).
- Krupnik, V.E., et al., Gene 238(2):301-313 (1999).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This DKK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 225-251 amino acids from the C-terminal region of human DKK2.
NCBI Gene ID #:
27123
NCBI Official Name:
Dickkopf-related protein 2
NCBI Official Symbol:
DKK2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
28 kDa
Protein Accession #:
Q9UBU2
Protein GI Number:
13124093
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q9UBU2
User NOte:
Optimal dilutions for each application to be determined by the researcher.