ARPC3 Antibody

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ProSci
Product Code: PSI-62-355
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-62-355-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of ARPC3 antibody in HL60 cell line lysates(35ug/lane)
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Formalin-fixed and paraffin-embedded human brain tissue reacted with ARPC3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of ARPC3 antibody in HL60 cell line lysates(35ug/lane)
Formalin-fixed and paraffin-embedded human brain tissue reacted with ARPC3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Documents

Further Information

Additional Names:
Actin-related protein 2/3 complex subunit 3, Arp2/3 complex 21 kDa subunit, p21-ARC, ARPC3, ARC21
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
ARPC3 is one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein, the p21 subunit, has yet to be determined.
Background References:
  • Dubois,T., Nat. Cell Biol.(4), 353-364 (2005)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This ARPC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 131-158 amino acids from the C-terminal region of human ARPC3.
NCBI Gene ID #:
10094
NCBI Official Name:
Actin-related protein 2/3 complex subunit 3
NCBI Official Symbol:
ARPC3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
21 kDa
Protein Accession #:
O15145
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction
Swissprot #:
O15145
User NOte:
Optimal dilutions for each application to be determined by the researcher.