DPT Antibody

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ProSci
Product Code: PSI-56-560
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-56-560-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of DPT using rabbit polyclonal DPT Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DPT gene.
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DPT Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis of DPT using rabbit polyclonal DPT Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DPT gene.
DPT Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Documents

Further Information

Additional Names:
Dermatopontin, Tyrosine-rich acidic matrix protein, TRAMP, DPT
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Dermatopontin is an extracellular matrix protein with possible functions in cell-matrix interactions and matrix assembly. The protein is found in various tissues and many of its tyrosine residues are sulphated. Dermatopontin is postulated to modify the behavior of TGF-beta through interaction with decorin. [provided by RefSeq].
Background References:
  • Ehret, G.B., et al. Eur. J. Hum. Genet. 17(12):1650-1657(2009)
  • Li, X., et al. Biochemistry Mosc. 74(9):979-985(2009)
  • Cheung, C.L., et al. Hum. Mol. Genet. 18(4):679-687(2009)
  • Pochampally, R.R., et al. J. Bone Miner. Res. 22(9):1338-1349(2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This DPT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 45-72 amino acids from the N-terminal region of human DPT.
NCBI Gene ID #:
1805
NCBI Official Name:
Dermatopontin
NCBI Official Symbol:
DPT
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
24 kDa
Protein Accession #:
Q07507
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction
Swissprot #:
Q07507
User NOte:
Optimal dilutions for each application to be determined by the researcher.