WIF1 Antibody

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ProSci
Product Code: PSI-61-453
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-61-453-400ul400ul£626.00
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of WIF1 using rabbit polyclonal WIF1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the WIF1 gene (Lane 2).
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Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with WIF1 (Human C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of WIF1 using rabbit polyclonal WIF1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the WIF1 gene (Lane 2).
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with WIF1 (Human C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Documents

Further Information

Additional Names:
Wnt inhibitory factor 1, WIF-1, WIF1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
WNT proteins are extracellular signaling molecules involved in the control of embryonic development. WIF1 is a secreted protein, which binds WNT proteins and inhibits their activities. This protein contains a WNT inhibitory factor (WIF) domain and 5 epidermal growth factor (EGF)-like domains. It may be involved in mesoderm segmentation. This protein is found to be present in fish, amphibia and mammals.
Background References:
  • Elston,M.S., Endocrinology 149 (3), 1235-1242 (2008)
  • Clement,G., Cancer Sci. 99 (1), 46-53 (2008)
  • Chan,S.L., Lab. Invest. 87 (7), 644-650 (2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Zebrafish, Mouse, Rat, Xenopus
Immunogen:
This WIF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-80 amino acids from the N-terminal region of human WIF1.
NCBI Gene ID #:
11197
NCBI Official Name:
Wnt inhibitory factor 1
NCBI Official Symbol:
WIF1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
42 kDa
Protein Accession #:
Q9Y5W5
Protein GI Number:
61252765
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q9Y5W5
User NOte:
Optimal dilutions for each application to be determined by the researcher.