RAB5C Antibody

ProSci
Product Code: PSI-57-052
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-57-052-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: 293 whole cell lysate Lysates/proteins at 20 ug per lane.
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RAB5C Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western Blot at 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: 293 whole cell lysate Lysates/proteins at 20 ug per lane.
RAB5C Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Ras-related protein Rab-5C, L1880, RAB5L, RAB5C, RABL
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Members of the Rab protein family are small GTPases of the Ras superfamily that are thought to ensure fidelity in the process of docking and/or fusion of vesicles with their correct acceptor compartment (Han et al., 1996 [PubMed 8646882]).
Background References:
  • Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
  • Chi, A., et al. J. Proteome Res. 5(11):3135-3144(2006)
  • Merithew, E., et al. J. Biol. Chem. 278(10):8494-8500(2003)
  • Clemens, D.L., et al. Infect. Immun. 68(5):2671-2684(2000)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This RAB5C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 178-207 amino acids from the C-terminal region of human RAB5C.
NCBI Gene ID #:
5878
NCBI Official Name:
Ras-related protein Rab-5C
NCBI Official Symbol:
RAB5C
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
23 kDa
Protein Accession #:
P51148
Protein GI Number:
38258923
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
P51148
User NOte:
Optimal dilutions for each application to be determined by the researcher.