SGK2 Antibody

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ProSci
Product Code: PSI-63-302
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-302-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of anti-SKG2 Pab in placenta lysate
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
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Western blot analysis of anti-SKG2 Pab in placenta lysate
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.

Documents

Further Information

Additional Names:
Serine/threonine-protein kinase Sgk2, Serum/glucocorticoid-regulated kinase 2, SGK2
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
SGK2, a Ser/Thr protein kinase, is similar to serum- and glucocorticoid-induced protein kinase (SGK), but this gene product is not induced by serum or glucocorticoids. Expression is induced in response to signals that activate phosphatidylinositol 3-kinase, which is also true for SGK.
Background References:
  • Friedrich, B., et al., Pflugers Arch. 445(6):693-696 (2003).
  • Embark, H.M., et al., Pflugers Arch. 445(5):601-606 (2003).
  • Gamper, N., et al., Pflugers Arch. 445(1):60-66 (2002).
  • Lang, F., et al., Sci. STKE 2001 (108), RE17 (2001).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This SGK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 395-426 amino acids from the C-terminal region of human SGK2.
NCBI Gene ID #:
10110
NCBI Official Name:
Serine/threonine-protein kinase Sgk2
NCBI Official Symbol:
SGK2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
48 kDa
Protein Accession #:
Q9HBY8
Protein GI Number:
28558166
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction
Swissprot #:
Q9HBY8
User NOte:
Optimal dilutions for each application to be determined by the researcher.