MYL9 Antibody

ProSci
Product Code: PSI-57-904
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-57-904-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in Jurkat cell line lysates (35ug/lane).
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MYL9 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis in Jurkat cell line lysates (35ug/lane).
MYL9 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Myosin regulatory light polypeptide 9, 20 kDa myosin light chain, LC20, MLC-2C, Myosin RLC, Myosin regulatory light chain 2, smooth muscle isoform, Myosin regulatory light chain 9, Myosin regulatory light chain MRLC1, MYL9, MLC2, MRLC1, MYRL2
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Myosin, a structural component of muscle, consists of two heavy chains and four light chains. The protein encoded by this gene is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. The encoded protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for this gene.
Background References:
  • Gilles, L., et al. Blood 114(19):4221-4232(2009)
  • Higashihara, M., et al.Smooth Muscle Res 44(1):29-40(2008)
  • Szczesna-Cordary, D., et al. J. Cell. Sci. 118 (PT 16), 3675-3683 (2005) :
  • Webb, R.C. Adv Physiol Educ 27 (1-4), 201-206 (2003) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Chicken, Mouse, Pig, Rat
Immunogen:
This MYL9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 96-125 amino acids from the C-terminal region of human MYL9.
NCBI Gene ID #:
10398
NCBI Official Name:
Myosin regulatory light polypeptide 9
NCBI Official Symbol:
MYL9
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
20 kDa
Protein Accession #:
P24844
Protein GI Number:
20141521
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
P24844
User NOte:
Optimal dilutions for each application to be determined by the researcher.