KRT35 Antibody

ProSci
Product Code: PSI-61-746
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-746-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of KRT35 Antibody in ZR-75-1 cell line lysates (35ug/lane)
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KRT35 Antibody IHC analysis in formalin fixed and paraffin embedded skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis of KRT35 Antibody in ZR-75-1 cell line lysates (35ug/lane)
KRT35 Antibody IHC analysis in formalin fixed and paraffin embedded skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Keratin, type I cuticular Ha5, Hair keratin, type I Ha5, Keratin-35, K35, KRT35, HHA5, HKA5, KRTHA5
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
KRT35 is a member of the keratin gene family. This type I hair keratin is an acidic protein which heterodimerizes with type II keratins to form hair and nails. The type I hair keratins are clustered in a region of chromosome 17q12-q21 and have the same direction of transcription.
Background References:
  • Gilon, M., et al. Differentiation 76(5):518-530(2008)
  • Schweizer, J., et al. J. Cell Biol. 174(2):169-174(2006)
  • Cribier, B., et al. J. Invest. Dermatol. 122(5):1078-1083(2004)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This KRT35 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 352-380 amino acids from the C-terminal region of human KRT35.
NCBI Gene ID #:
3886
NCBI Official Name:
Keratin, type I cuticular Ha5
NCBI Official Symbol:
KRT35
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
50 kDa
Protein Accession #:
Q92764
Protein GI Number:
296439499
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q92764
User NOte:
Optimal dilutions for each application to be determined by the researcher.